Labeling:Article Title: Methods for the identification of microRNA and their applications in research and human health
Article Snippet: .. Buffers 10 × TBE buffer, pH 8.0 at 25° C. Tris 890 mM Boric acid 890 mM EDTA 20 mM Denaturing Polyacrylamide Gel Polyacrylamide 15% Urea 7M Ammonium Persulfat 0.1% TEMED 25 μl/100 ml in 1 × TBE Sample buffer Formamide 95% 500 mM EDTA 20 mM Bromophenol Blue 0.05% Xylene Cyanol FF 0.05% Elution buffer Sodium Acetate 500 mM EDTA 1 mM Sample Labeling Samples were labeled Cy3 and/or Cy5 using the mirVanaTM miRNA labeling kit from Ambion Microarray Probe Design The oligonucleotides were designed according to SEQ ID NOs: 77, 139, 223, 328, 728, 832, 858, 951, 963, 1083, 1173, 1205, 1429, 1611, 1617, 1649, 1688 and to A. thaliana microRNA sequence named ath-MIR156a, ath-MIR157 as negative control (the precursor sequence of both are not present in the human genome). ..
Microarray:Article Title: Methods for the identification of microRNA and their applications in research and human health
Article Snippet: .. Buffers 10 × TBE buffer, pH 8.0 at 25° C. Tris 890 mM Boric acid 890 mM EDTA 20 mM Denaturing Polyacrylamide Gel Polyacrylamide 15% Urea 7M Ammonium Persulfat 0.1% TEMED 25 μl/100 ml in 1 × TBE Sample buffer Formamide 95% 500 mM EDTA 20 mM Bromophenol Blue 0.05% Xylene Cyanol FF 0.05% Elution buffer Sodium Acetate 500 mM EDTA 1 mM Sample Labeling Samples were labeled Cy3 and/or Cy5 using the mirVanaTM miRNA labeling kit from Ambion Microarray Probe Design The oligonucleotides were designed according to SEQ ID NOs: 77, 139, 223, 328, 728, 832, 858, 951, 963, 1083, 1173, 1205, 1429, 1611, 1617, 1649, 1688 and to A. thaliana microRNA sequence named ath-MIR156a, ath-MIR157 as negative control (the precursor sequence of both are not present in the human genome). ..
Sequencing:Article Title: Methods for the identification of microRNA and their applications in research and human health
Article Snippet: .. Buffers 10 × TBE buffer, pH 8.0 at 25° C. Tris 890 mM Boric acid 890 mM EDTA 20 mM Denaturing Polyacrylamide Gel Polyacrylamide 15% Urea 7M Ammonium Persulfat 0.1% TEMED 25 μl/100 ml in 1 × TBE Sample buffer Formamide 95% 500 mM EDTA 20 mM Bromophenol Blue 0.05% Xylene Cyanol FF 0.05% Elution buffer Sodium Acetate 500 mM EDTA 1 mM Sample Labeling Samples were labeled Cy3 and/or Cy5 using the mirVanaTM miRNA labeling kit from Ambion Microarray Probe Design The oligonucleotides were designed according to SEQ ID NOs: 77, 139, 223, 328, 728, 832, 858, 951, 963, 1083, 1173, 1205, 1429, 1611, 1617, 1649, 1688 and to A. thaliana microRNA sequence named ath-MIR156a, ath-MIR157 as negative control (the precursor sequence of both are not present in the human genome). ..
Negative Control:Article Title: Methods for the identification of microRNA and their applications in research and human health
Article Snippet: .. Buffers 10 × TBE buffer, pH 8.0 at 25° C. Tris 890 mM Boric acid 890 mM EDTA 20 mM Denaturing Polyacrylamide Gel Polyacrylamide 15% Urea 7M Ammonium Persulfat 0.1% TEMED 25 μl/100 ml in 1 × TBE Sample buffer Formamide 95% 500 mM EDTA 20 mM Bromophenol Blue 0.05% Xylene Cyanol FF 0.05% Elution buffer Sodium Acetate 500 mM EDTA 1 mM Sample Labeling Samples were labeled Cy3 and/or Cy5 using the mirVanaTM miRNA labeling kit from Ambion Microarray Probe Design The oligonucleotides were designed according to SEQ ID NOs: 77, 139, 223, 328, 728, 832, 858, 951, 963, 1083, 1173, 1205, 1429, 1611, 1617, 1649, 1688 and to A. thaliana microRNA sequence named ath-MIR156a, ath-MIR157 as negative control (the precursor sequence of both are not present in the human genome). ..
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